2/16/2023 0 Comments Online primer design tool![]() ![]() tool for identifying simple sequence repeat (SSR) loci by analysing the low complexity regions of input sequences. ![]() tool calculate Tm for primer dimers with mismatches for pure and mixed bases using averaged nearest neighbour thermodynamic parameters and for modifications (inosine, uridine, or LNA).primers (probes) are analyzed for all primer secondary structures including the alternative hydrogen bonding to Watson-Crick base pairing such as G-quadruplexes or wobble base pairs (like G-G, G-T, G-A), self-dimers and cross-dimers in primer pairs.analyzes different features of multiple primers simultaneously, the melting temperature, GC content, sequence linguistic complexity, primer PCR efficiency and molecular weight, the extinction coefficient, the optical density (OD).comprehensive primer test, the melting temperature calculation for standard and degenerate oligonucleotides including LNA and other modifications, primer's PCR efficiency and linguistic complexity, and dilution and resuspension calculator.in silico (virtual) PCR for primers and probes searching or in silico PCR against genome(s) (or a list of chromosome) - prediction of probable PCR products and search of potential mismatching location of the specified primers or probes. ![]()
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